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1x phosphate buffered saline  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 1x phosphate buffered saline
    1x Phosphate Buffered Saline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sterile+phosphate+buffered+saline/Phosphate-buffered+saline+(PBS%2C+1X)%2C+sterile-filtered/pmc13088972-371-15-21
    Average 99 stars, based on 1 article reviews
    1x phosphate buffered saline - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Immunomodulatory topographies regulate myofibroblast differentiation and influence fibrous encapsulation of glaucoma drainage devices
    Article Snippet: .. After cell culture, TopoChips or large topographical surfaces were washed three times with sterile phosphate-buffered saline (PBS; Gibco) and fixed with 3.7 % (v/v) paraformaldehyde (Sigma- Aldrich) for 15 min at room temperature. .. Next, the cells were permeabilized with 0.5 % Triton X-100 (Merck) dissolved in PBS for 10 min and blocked with 3 % (w/v) bovine serum albumin (Merck) in PBS for 30 min and then with 5 % (v/v) goat serum (Fisher Scientific) dissolved in PBS for 30 min. Next, cells were incubated with a primary antibody using mouse monoclonal anti α-smooth muscle actin diluted in PBS (1:600; Sigma: A5228) overnight at 4 °C.

    Sterility:

    Article Title: Immunomodulatory topographies regulate myofibroblast differentiation and influence fibrous encapsulation of glaucoma drainage devices
    Article Snippet: .. After cell culture, TopoChips or large topographical surfaces were washed three times with sterile phosphate-buffered saline (PBS; Gibco) and fixed with 3.7 % (v/v) paraformaldehyde (Sigma- Aldrich) for 15 min at room temperature. .. Next, the cells were permeabilized with 0.5 % Triton X-100 (Merck) dissolved in PBS for 10 min and blocked with 3 % (w/v) bovine serum albumin (Merck) in PBS for 30 min and then with 5 % (v/v) goat serum (Fisher Scientific) dissolved in PBS for 30 min. Next, cells were incubated with a primary antibody using mouse monoclonal anti α-smooth muscle actin diluted in PBS (1:600; Sigma: A5228) overnight at 4 °C.

    Article Title: Anticancer Effects of Clausena hamandiana: Ethanolic Extract Inhibits Cancer Cell Proliferation and Suppresses Lung Tumorigenesis in Mice
    Article Snippet: Murine lung carcinoma 3LL–Luciferase2 (3LL–Luc2) cells (PerkinElmer, Waltham, MA, USA) were cultured under standard conditions in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin–streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). .. Cells were prepared at a concentration of 1 × 105 cells/mL in sterile phosphate-buffered saline (PBS; Gibco, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Effects of empagliflozin and metformin on biofilm formation and pathogenicity factors of urinary Escherichia coli isolates.
    Article Snippet: .. The well contents were gently aspirated and washed with sterile phosphate-buffered saline (Oxoid, UK) to remove non-adherent cells. ..

    Article Title: Structural basis for prohibitin-mediated regulation of mitochondrial m-AAA protease.
    Article Snippet: .. Cell suspensions were harvested, washed with sterile phosphate-buffered saline (PBS, Thermo Fisher Scientific), and normalized to equal cell densities based on OD600. ..

    Article Title: DAL, lectin from Dioclea altissima: Three-dimensional structure resolution by X-ray diffraction and in silico prospection for targeting Mpro from SARS-CoV-2.
    Article Snippet: Plant lectins are carbohydrate-binding proteins with diverse biological functions and growing biomedical relevance due to their ability to recognize specific glycan structures.. Here, we report the high-resolution crystallographic structure of a lectin from D. altissima seeds (DAL), resolved by X-ray diffraction at 2.21 Å (PDB ID: 7LJG).. The structure reveals a homodimeric fold consistent with other lectins from the Dioclea genus, reinforcing its conserved molecular architecture.

    Article Title: Modulation of angiogenic, inflammatory, and matrix remodeling responses in type 2 diabetic wounds by a decellularized dermal scaffold and adjunct biophysical stimulation.
    Article Snippet: Impaired wound healing in type 2 diabetes is largely attributed to dysregulated cellular responses, defective extracellular matrix (ECM) remodeling, insufficient angiogenesis, and a prolonged inflammatory microenvironment.. Understanding how these processes can be modulated at the cellular and tissue levels remains essential for improving diabetic wound repair.. In this study, we examined the effects of a decellularized dermal scaffold (DDS) combined with photobiomodulation therapy (PBM), applied as an adjunct biophysical stimulus, on angiogenic, inflammatory, and matrix remodeling responses in a type 2 diabetic rat wound model. Full-thickness excisional skin wounds were created and assigned to control, DDS-treated, PBM-treated, or combined DDS + PBM groups.

    Saline:

    Article Title: Immunomodulatory topographies regulate myofibroblast differentiation and influence fibrous encapsulation of glaucoma drainage devices
    Article Snippet: .. After cell culture, TopoChips or large topographical surfaces were washed three times with sterile phosphate-buffered saline (PBS; Gibco) and fixed with 3.7 % (v/v) paraformaldehyde (Sigma- Aldrich) for 15 min at room temperature. .. Next, the cells were permeabilized with 0.5 % Triton X-100 (Merck) dissolved in PBS for 10 min and blocked with 3 % (w/v) bovine serum albumin (Merck) in PBS for 30 min and then with 5 % (v/v) goat serum (Fisher Scientific) dissolved in PBS for 30 min. Next, cells were incubated with a primary antibody using mouse monoclonal anti α-smooth muscle actin diluted in PBS (1:600; Sigma: A5228) overnight at 4 °C.

    Article Title: Anticancer Effects of Clausena hamandiana: Ethanolic Extract Inhibits Cancer Cell Proliferation and Suppresses Lung Tumorigenesis in Mice
    Article Snippet: Murine lung carcinoma 3LL–Luciferase2 (3LL–Luc2) cells (PerkinElmer, Waltham, MA, USA) were cultured under standard conditions in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin–streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). .. Cells were prepared at a concentration of 1 × 105 cells/mL in sterile phosphate-buffered saline (PBS; Gibco, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Effects of empagliflozin and metformin on biofilm formation and pathogenicity factors of urinary Escherichia coli isolates.
    Article Snippet: .. The well contents were gently aspirated and washed with sterile phosphate-buffered saline (Oxoid, UK) to remove non-adherent cells. ..

    Article Title: Structural basis for prohibitin-mediated regulation of mitochondrial m-AAA protease.
    Article Snippet: .. Cell suspensions were harvested, washed with sterile phosphate-buffered saline (PBS, Thermo Fisher Scientific), and normalized to equal cell densities based on OD600. ..

    Article Title: DAL, lectin from Dioclea altissima: Three-dimensional structure resolution by X-ray diffraction and in silico prospection for targeting Mpro from SARS-CoV-2.
    Article Snippet: Plant lectins are carbohydrate-binding proteins with diverse biological functions and growing biomedical relevance due to their ability to recognize specific glycan structures.. Here, we report the high-resolution crystallographic structure of a lectin from D. altissima seeds (DAL), resolved by X-ray diffraction at 2.21 Å (PDB ID: 7LJG).. The structure reveals a homodimeric fold consistent with other lectins from the Dioclea genus, reinforcing its conserved molecular architecture.

    Article Title: Modulation of angiogenic, inflammatory, and matrix remodeling responses in type 2 diabetic wounds by a decellularized dermal scaffold and adjunct biophysical stimulation.
    Article Snippet: Impaired wound healing in type 2 diabetes is largely attributed to dysregulated cellular responses, defective extracellular matrix (ECM) remodeling, insufficient angiogenesis, and a prolonged inflammatory microenvironment.. Understanding how these processes can be modulated at the cellular and tissue levels remains essential for improving diabetic wound repair.. In this study, we examined the effects of a decellularized dermal scaffold (DDS) combined with photobiomodulation therapy (PBM), applied as an adjunct biophysical stimulus, on angiogenic, inflammatory, and matrix remodeling responses in a type 2 diabetic rat wound model. Full-thickness excisional skin wounds were created and assigned to control, DDS-treated, PBM-treated, or combined DDS + PBM groups.

    Concentration Assay:

    Article Title: Anticancer Effects of Clausena hamandiana: Ethanolic Extract Inhibits Cancer Cell Proliferation and Suppresses Lung Tumorigenesis in Mice
    Article Snippet: Murine lung carcinoma 3LL–Luciferase2 (3LL–Luc2) cells (PerkinElmer, Waltham, MA, USA) were cultured under standard conditions in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin–streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). .. Cells were prepared at a concentration of 1 × 105 cells/mL in sterile phosphate-buffered saline (PBS; Gibco, Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: DAL, lectin from Dioclea altissima: Three-dimensional structure resolution by X-ray diffraction and in silico prospection for targeting Mpro from SARS-CoV-2.
    Article Snippet: Plant lectins are carbohydrate-binding proteins with diverse biological functions and growing biomedical relevance due to their ability to recognize specific glycan structures.. Here, we report the high-resolution crystallographic structure of a lectin from D. altissima seeds (DAL), resolved by X-ray diffraction at 2.21 Å (PDB ID: 7LJG).. The structure reveals a homodimeric fold consistent with other lectins from the Dioclea genus, reinforcing its conserved molecular architecture.

    Isolation:

    Article Title: DAL, lectin from Dioclea altissima: Three-dimensional structure resolution by X-ray diffraction and in silico prospection for targeting Mpro from SARS-CoV-2.
    Article Snippet: Plant lectins are carbohydrate-binding proteins with diverse biological functions and growing biomedical relevance due to their ability to recognize specific glycan structures.. Here, we report the high-resolution crystallographic structure of a lectin from D. altissima seeds (DAL), resolved by X-ray diffraction at 2.21 Å (PDB ID: 7LJG).. The structure reveals a homodimeric fold consistent with other lectins from the Dioclea genus, reinforcing its conserved molecular architecture.

    other:

    Article Title: Influence of Fluoride-Doped Calcium Phosphates on the Mineralisation, Physicochemical Properties and Microbiological Activity of Glass Ionomer Cements.
    Article Snippet: 2.14 Evaluation of cytotoxicity on human gingival fibroblast Human gingival fibroblasts (HGFs, ATCC PCS-201-018) at passage 4 were cultured in T75 cell culture flasks with Dulbecco's Modified Eagle Medium (ATCC PCS-201-030, Gibco, Life Technologies, Carlsbad, United States), and 1% Penicillin-Streptomycin (Penstrep, Gibco, Life Technologies, Carlsbad, United States) at 37°C in a humidified incubator with 5% CO2.



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